Construct: shRNA TRCN0000064341

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000782.2-1395s1c1
DNA Barcode:
GCAAACAGTCTAATGTGGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CYP24A1 (1591)
Gene Description:
cytochrome P450 family 24 subfamily A member 1
Transcript:
RefSeq NM_000782.2 (NON-CURRENT)
Match location:
Position 1395 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000064341)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAACAGTCTAATGTGGATT
Hairpin Sequence:
5'-CCGG-GCAAACAGTCTAATGTGGATT-CTCGAG-AATCCACATTAGACTGTTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAACAGTCTAATGTGGATTCTCGAGAATCCACATTAGACTGTTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAACAGTCTAATGTGGATTCTCGAGAATCCACATTAGACTGTTTGC-3'

Other clones with same target sequence:

(none)