Construct: shRNA TRCN0000064396

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032709.1-1016s1c1
DNA Barcode:
CAAGGAGTTGTGCTGGAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PYROXD2 (84795)
Gene Description:
pyridine nucleotide-disulphide oxidoreductase domain 2
Transcript:
RefSeq NM_032709.1 (NON-CURRENT)
Match location:
Position 1016 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000064396)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGGAGTTGTGCTGGAAGAT
Hairpin Sequence:
5'-CCGG-CAAGGAGTTGTGCTGGAAGAT-CTCGAG-ATCTTCCAGCACAACTCCTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGGAGTTGTGCTGGAAGATCTCGAGATCTTCCAGCACAACTCCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGGAGTTGTGCTGGAAGATCTCGAGATCTTCCAGCACAACTCCTTG-3'

Other clones with same target sequence:

(none)