Construct: shRNA TRCN0000064432

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001985.1-123s1c1
DNA Barcode:
TGGTGTGAAGCACTCCATGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ETFB (2109)
Gene Description:
electron transfer flavoprotein subunit beta
Transcript:
RefSeq NM_001985.1 (NON-CURRENT)
Match location:
Position 123 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000064432)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGTGTGAAGCACTCCATGAA
Hairpin Sequence:
5'-CCGG-TGGTGTGAAGCACTCCATGAA-CTCGAG-TTCATGGAGTGCTTCACACCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGTGTGAAGCACTCCATGAACTCGAGTTCATGGAGTGCTTCACACCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGTGTGAAGCACTCCATGAACTCGAGTTCATGGAGTGCTTCACACCA-3'

Other clones with same target sequence:

  1. TRCN0000291142