Construct: shRNA TRCN0000064510

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001461.1-827s1c1
DNA Barcode:
GCCAATCATTAGCAAACAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FMO5 (2330)
Gene Description:
flavin containing dimethylaniline monoxygenase 5
Transcript:
RefSeq NM_001461.1 (NON-CURRENT)
Match location:
Position 827 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000064510)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAATCATTAGCAAACAAAT
Hairpin Sequence:
5'-CCGG-GCCAATCATTAGCAAACAAAT-CTCGAG-ATTTGTTTGCTAATGATTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAATCATTAGCAAACAAATCTCGAGATTTGTTTGCTAATGATTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAATCATTAGCAAACAAATCTCGAGATTTGTTTGCTAATGATTGGC-3'

Other clones with same target sequence:

(none)