Construct: shRNA TRCN0000064538

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004820.2-895s1c1
DNA Barcode:
CCCATTGAGCTTCTAGGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CYP7B1 (9420)
Gene Description:
cytochrome P450 family 7 subfamily B member 1
Transcript:
RefSeq NM_004820.2 (NON-CURRENT)
Match location:
Position 895 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000064538)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATTGAGCTTCTAGGAAAT
Hairpin Sequence:
5'-CCGG-CCCATTGAGCTTCTAGGAAAT-CTCGAG-ATTTCCTAGAAGCTCAATGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATTGAGCTTCTAGGAAATCTCGAGATTTCCTAGAAGCTCAATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATTGAGCTTCTAGGAAATCTCGAGATTTCCTAGAAGCTCAATGGG-3'

Other clones with same target sequence:

(none)