Construct: shRNA TRCN0000064618

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000499.2-822s1c1
DNA Barcode:
GCTGACTTCATCCCTATTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CYP1A1 (1543)
Gene Description:
cytochrome P450 family 1 subfamily A member 1
Transcript:
RefSeq NM_000499.2 (NON-CURRENT)
Match location:
Position 822 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000064618)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGACTTCATCCCTATTCTT
Hairpin Sequence:
5'-CCGG-GCTGACTTCATCCCTATTCTT-CTCGAG-AAGAATAGGGATGAAGTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGACTTCATCCCTATTCTTCTCGAGAAGAATAGGGATGAAGTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGACTTCATCCCTATTCTTCTCGAGAAGAATAGGGATGAAGTCAGC-3'

Other clones with same target sequence:

(none)