Construct: shRNA TRCN0000064624

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178134.2-414s1c1
DNA Barcode:
CCAGATTGTGAAACCTGGCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CYP4Z1 (199974)
Gene Description:
cytochrome P450 family 4 subfamily Z member 1
Transcript:
RefSeq NM_178134.2 (NON-CURRENT)
Match location:
Position 414 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000064624)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGATTGTGAAACCTGGCTT
Hairpin Sequence:
5'-CCGG-CCAGATTGTGAAACCTGGCTT-CTCGAG-AAGCCAGGTTTCACAATCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGATTGTGAAACCTGGCTTCTCGAGAAGCCAGGTTTCACAATCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGATTGTGAAACCTGGCTTCTCGAGAAGCCAGGTTTCACAATCTGG-3'

Other clones with same target sequence:

(none)