Construct: shRNA TRCN0000064653

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000762.4-342s1c1
DNA Barcode:
CAAAGGCTATGGCGTGGTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CYP2A6 (1548)
Gene Description:
cytochrome P450 family 2 subfamily A member 6
Transcript:
RefSeq NM_000762.4 (NON-CURRENT)
Match location:
Position 342 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000064653)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAAGGCTATGGCGTGGTATT
Hairpin Sequence:
5'-CCGG-CAAAGGCTATGGCGTGGTATT-CTCGAG-AATACCACGCCATAGCCTTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAAGGCTATGGCGTGGTATTCTCGAGAATACCACGCCATAGCCTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAAGGCTATGGCGTGGTATTCTCGAGAATACCACGCCATAGCCTTTG-3'

Other clones with same target sequence:

(none)