Construct: shRNA TRCN0000064686

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_206918.1-101s1c1
DNA Barcode:
GCGCAAGGAGATACTGGCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DEGS2 (123099)
Gene Description:
delta 4-desaturase, sphingolipid 2
Transcript:
RefSeq NM_206918.1 (NON-CURRENT)
Match location:
Position 101 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000064686)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCAAGGAGATACTGGCCAA
Hairpin Sequence:
5'-CCGG-GCGCAAGGAGATACTGGCCAA-CTCGAG-TTGGCCAGTATCTCCTTGCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCAAGGAGATACTGGCCAACTCGAGTTGGCCAGTATCTCCTTGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCAAGGAGATACTGGCCAACTCGAGTTGGCCAGTATCTCCTTGCGC-3'

Other clones with same target sequence:

(none)