Construct: shRNA TRCN0000064750

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139178.1-674s1c1
DNA Barcode:
GTCTGTTTGTATCCTGGCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ALKBH3 (221120)
Gene Description:
alkB homolog 3, alpha-ketoglutaratedependent dioxygenase
Transcript:
RefSeq NM_139178.1 (NON-CURRENT)
Match location:
Position 674 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000064750)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTGTTTGTATCCTGGCTTT
Hairpin Sequence:
5'-CCGG-GTCTGTTTGTATCCTGGCTTT-CTCGAG-AAAGCCAGGATACAAACAGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTGTTTGTATCCTGGCTTTCTCGAGAAAGCCAGGATACAAACAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTGTTTGTATCCTGGCTTTCTCGAGAAAGCCAGGATACAAACAGAC-3'

Other clones with same target sequence:

(none)