Construct: shRNA TRCN0000064752

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139178.1-1182s1c1
DNA Barcode:
TGCCCAAAGAATACCACTCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ALKBH3 (221120)
Gene Description:
alkB homolog 3, alpha-ketoglutaratedependent dioxygenase
Transcript:
RefSeq NM_139178.1 (NON-CURRENT)
Match location:
Position 1182 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000064752)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCCCAAAGAATACCACTCTA
Hairpin Sequence:
5'-CCGG-TGCCCAAAGAATACCACTCTA-CTCGAG-TAGAGTGGTATTCTTTGGGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCCCAAAGAATACCACTCTACTCGAGTAGAGTGGTATTCTTTGGGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCCCAAAGAATACCACTCTACTCGAGTAGAGTGGTATTCTTTGGGCA-3'

Other clones with same target sequence:

(none)