Construct: shRNA TRCN0000064755

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004265.2-456s1c1
DNA Barcode:
CCACGGCAAGAACTCAAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FADS2 (9415)
Gene Description:
fatty acid desaturase 2
Transcript:
RefSeq NM_004265.2 (NON-CURRENT)
Match location:
Position 456 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000064755)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACGGCAAGAACTCAAAGAT
Hairpin Sequence:
5'-CCGG-CCACGGCAAGAACTCAAAGAT-CTCGAG-ATCTTTGAGTTCTTGCCGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACGGCAAGAACTCAAAGATCTCGAGATCTTTGAGTTCTTGCCGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACGGCAAGAACTCAAAGATCTCGAGATCTTTGAGTTCTTGCCGTGG-3'

Other clones with same target sequence:

  1. TRCN0000291763