Construct: shRNA TRCN0000064797

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018192.2-997s1c1
DNA Barcode:
GCTGGTCTGTATGAAGCTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
P3H2 (55214)
Gene Description:
prolyl 3-hydroxylase 2
Transcript:
RefSeq NM_018192.2 (NON-CURRENT)
Match location:
Position 997 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000064797)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGTCTGTATGAAGCTATT
Hairpin Sequence:
5'-CCGG-GCTGGTCTGTATGAAGCTATT-CTCGAG-AATAGCTTCATACAGACCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGTCTGTATGAAGCTATTCTCGAGAATAGCTTCATACAGACCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGTCTGTATGAAGCTATTCTCGAGAATAGCTTCATACAGACCAGC-3'

Other clones with same target sequence:

  1. TRCN0000291842