Construct: shRNA TRCN0000064809

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000935.1-1894s1c1
DNA Barcode:
CCAGTTACACTGAAGGTCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLOD2 (5352)
Gene Description:
procollagen-lysine,2-oxoglutarate 5-dioxygenase 2
Transcript:
RefSeq NM_000935.1 (NON-CURRENT)
Match location:
Position 1894 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000064809)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTTACACTGAAGGTCTTT
Hairpin Sequence:
5'-CCGG-CCAGTTACACTGAAGGTCTTT-CTCGAG-AAAGACCTTCAGTGTAACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTTACACTGAAGGTCTTTCTCGAGAAAGACCTTCAGTGTAACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTTACACTGAAGGTCTTTCTCGAGAAAGACCTTCAGTGTAACTGG-3'

Other clones with same target sequence:

(none)