Construct: shRNA TRCN0000064836

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006680.1-1774s1c1
DNA Barcode:
CTCCAGACTATGACTCCTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ME3 (10873)
Gene Description:
malic enzyme 3
Transcript:
RefSeq NM_006680.1 (NON-CURRENT)
Match location:
Position 1774 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000064836)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCCAGACTATGACTCCTTTA
Hairpin Sequence:
5'-CCGG-CTCCAGACTATGACTCCTTTA-CTCGAG-TAAAGGAGTCATAGTCTGGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCCAGACTATGACTCCTTTACTCGAGTAAAGGAGTCATAGTCTGGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCCAGACTATGACTCCTTTACTCGAGTAAAGGAGTCATAGTCTGGAG-3'

Other clones with same target sequence:

(none)