Construct: shRNA TRCN0000064843

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020175.1-445s1c1
DNA Barcode:
GCTAAGTGTTTCTTCGGTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DUS3L (56931)
Gene Description:
dihydrouridine synthase 3 like
Transcript:
RefSeq NM_020175.1 (NON-CURRENT)
Match location:
Position 445 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000064843)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAAGTGTTTCTTCGGTGAT
Hairpin Sequence:
5'-CCGG-GCTAAGTGTTTCTTCGGTGAT-CTCGAG-ATCACCGAAGAAACACTTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAAGTGTTTCTTCGGTGATCTCGAGATCACCGAAGAAACACTTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAAGTGTTTCTTCGGTGATCTCGAGATCACCGAAGAAACACTTAGC-3'

Other clones with same target sequence:

  1. TRCN0000349131