Construct: shRNA TRCN0000064846

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020175.1-946s1c1
DNA Barcode:
CGGCTGGACATCCGTGGCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DUS3L (56931)
Gene Description:
dihydrouridine synthase 3 like
Transcript:
RefSeq NM_020175.1 (NON-CURRENT)
Match location:
Position 946 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000064846)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCTGGACATCCGTGGCAAA
Hairpin Sequence:
5'-CCGG-CGGCTGGACATCCGTGGCAAA-CTCGAG-TTTGCCACGGATGTCCAGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCTGGACATCCGTGGCAAACTCGAGTTTGCCACGGATGTCCAGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCTGGACATCCGTGGCAAACTCGAGTTTGCCACGGATGTCCAGCCG-3'

Other clones with same target sequence:

  1. TRCN0000315797