Construct: shRNA TRCN0000064898

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005651.1-688s1c1
DNA Barcode:
GCTGGAAAGAACTCCAGGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TDO2 (6999)
Gene Description:
tryptophan 2,3-dioxygenase
Transcript:
RefSeq NM_005651.1 (NON-CURRENT)
Match location:
Position 688 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000064898)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGAAAGAACTCCAGGTTT
Hairpin Sequence:
5'-CCGG-GCTGGAAAGAACTCCAGGTTT-CTCGAG-AAACCTGGAGTTCTTTCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGAAAGAACTCCAGGTTTCTCGAGAAACCTGGAGTTCTTTCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGAAAGAACTCCAGGTTTCTCGAGAAACCTGGAGTTCTTTCCAGC-3'

Other clones with same target sequence:

(none)