Construct: shRNA TRCN0000064910

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001148.2-11485s1c1
DNA Barcode:
CCCATCATACAAGAACCCGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANK2 (287)
Gene Description:
ankyrin 2
Transcript:
RefSeq NM_001148.2 (NON-CURRENT)
Match location:
Position 11485 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000064910)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATCATACAAGAACCCGAA
Hairpin Sequence:
5'-CCGG-CCCATCATACAAGAACCCGAA-CTCGAG-TTCGGGTTCTTGTATGATGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATCATACAAGAACCCGAACTCGAGTTCGGGTTCTTGTATGATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATCATACAAGAACCCGAACTCGAGTTCGGGTTCTTGTATGATGGG-3'

Other clones with same target sequence:

(none)