Construct: shRNA TRCN0000064995

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003100.2-150s1c1
DNA Barcode:
CCCTAGAGTCAAGTCCATCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SNX2 (6643)
Gene Description:
sorting nexin 2
Transcript:
RefSeq NM_003100.2 (NON-CURRENT)
Match location:
Position 150 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000064995)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTAGAGTCAAGTCCATCAT
Hairpin Sequence:
5'-CCGG-CCCTAGAGTCAAGTCCATCAT-CTCGAG-ATGATGGACTTGACTCTAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTAGAGTCAAGTCCATCATCTCGAGATGATGGACTTGACTCTAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTAGAGTCAAGTCCATCATCTCGAGATGATGGACTTGACTCTAGGG-3'

Other clones with same target sequence:

  1. TRCN0000299056