Construct: shRNA TRCN0000065006

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015044.2-1365s1c1
DNA Barcode:
CCGTGCCCTCTGAATTATGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GGA2 (23062)
Gene Description:
golgi associated, gamma adaptin ear containing, ARF binding protein 2
Transcript:
RefSeq NM_015044.2 (NON-CURRENT)
Match location:
Position 1365 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000065006)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTGCCCTCTGAATTATGTT
Hairpin Sequence:
5'-CCGG-CCGTGCCCTCTGAATTATGTT-CTCGAG-AACATAATTCAGAGGGCACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTGCCCTCTGAATTATGTTCTCGAGAACATAATTCAGAGGGCACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTGCCCTCTGAATTATGTTCTCGAGAACATAATTCAGAGGGCACGG-3'

Other clones with same target sequence:

  1. TRCN0000286090