Construct: shRNA TRCN0000065029

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007364.1-372s1c1
DNA Barcode:
GCTGAAGTCAAGGGCGTTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMED3 (23423)
Gene Description:
transmembrane p24 trafficking protein 3
Transcript:
RefSeq NM_007364.1 (NON-CURRENT)
Match location:
Position 372 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000065029)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAAGTCAAGGGCGTTTAT
Hairpin Sequence:
5'-CCGG-GCTGAAGTCAAGGGCGTTTAT-CTCGAG-ATAAACGCCCTTGACTTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAAGTCAAGGGCGTTTATCTCGAGATAAACGCCCTTGACTTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAAGTCAAGGGCGTTTATCTCGAGATAAACGCCCTTGACTTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000298996