Construct: shRNA TRCN0000065122

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016040.3-850s1c1
DNA Barcode:
GAACAAGAAGATTGGAAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMED5 (50999)
Gene Description:
transmembrane p24 trafficking protein 5
Transcript:
RefSeq NM_016040.3 (NON-CURRENT)
Match location:
Position 850 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000065122)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACAAGAAGATTGGAAGAAA
Hairpin Sequence:
5'-CCGG-GAACAAGAAGATTGGAAGAAA-CTCGAG-TTTCTTCCAATCTTCTTGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACAAGAAGATTGGAAGAAACTCGAGTTTCTTCCAATCTTCTTGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACAAGAAGATTGGAAGAAACTCGAGTTTCTTCCAATCTTCTTGTTC-3'

Other clones with same target sequence:

  1. TRCN0000289694