Construct: shRNA TRCN0000065136

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001127.2-1644s1c1
DNA Barcode:
CCATTGTGAAACTCTTTCTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AP1B1 (162)
Gene Description:
adaptor related protein complex 1 subunit beta 1
Transcript:
RefSeq NM_001127.2 (NON-CURRENT)
Match location:
Position 1644 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000065136)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATTGTGAAACTCTTTCTAA
Hairpin Sequence:
5'-CCGG-CCATTGTGAAACTCTTTCTAA-CTCGAG-TTAGAAAGAGTTTCACAATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATTGTGAAACTCTTTCTAACTCGAGTTAGAAAGAGTTTCACAATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATTGTGAAACTCTTTCTAACTCGAGTTAGAAAGAGTTTCACAATGG-3'

Other clones with same target sequence:

(none)