Construct: shRNA TRCN0000065169

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014822.1-1383s1c1
DNA Barcode:
CCACCATTCTATTTCCAACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SEC24D (9871)
Gene Description:
SEC24 homolog D, COPII coat complex component
Transcript:
RefSeq NM_014822.1 (NON-CURRENT)
Match location:
Position 1383 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000065169)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCATTCTATTTCCAACAT
Hairpin Sequence:
5'-CCGG-CCACCATTCTATTTCCAACAT-CTCGAG-ATGTTGGAAATAGAATGGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCATTCTATTTCCAACATCTCGAGATGTTGGAAATAGAATGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCATTCTATTTCCAACATCTCGAGATGTTGGAAATAGAATGGTGG-3'

Other clones with same target sequence:

(none)