Construct: shRNA TRCN0000065268
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_004371.2-3637s1c1
- DNA Barcode:
- CCGTGAGTACATTGTGGGTTT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- COPA (1314)
- Gene Description:
- COPI coat complex subunit alpha
- Transcript:
- RefSeq NM_004371.2 (NON-CURRENT)
- Match location:
- Position 3637 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000065268)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCGTGAGTACATTGTGGGTTT
- Hairpin Sequence:
-
5'-CCGG-CCGTGAGTACATTGTGGGTTT-CTCGAG-AAACCCACAATGTACTCACGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCGTGAGTACATTGTGGGTTTCTCGAGAAACCCACAATGTACTCACGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCGTGAGTACATTGTGGGTTTCTCGAGAAACCCACAATGTACTCACGG-3'
Other clones with same target sequence:
-
TRCN0000289246
