Construct: shRNA TRCN0000065288

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006803.2-3231s1c1
DNA Barcode:
CCTAAGAAGATATAGAGTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AP3M2 (10947)
Gene Description:
adaptor related protein complex 3 subunit mu 2
Transcript:
RefSeq NM_006803.2 (NON-CURRENT)
Match location:
Position 3231 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000065288)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAAGAAGATATAGAGTGTT
Hairpin Sequence:
5'-CCGG-CCTAAGAAGATATAGAGTGTT-CTCGAG-AACACTCTATATCTTCTTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAAGAAGATATAGAGTGTTCTCGAGAACACTCTATATCTTCTTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAAGAAGATATAGAGTGTTCTCGAGAACACTCTATATCTTCTTAGG-3'

Other clones with same target sequence:

(none)