Construct: shRNA TRCN0000065289

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006803.2-1263s1c1
DNA Barcode:
CGTCTGGATATGTATGGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AP3M2 (10947)
Gene Description:
adaptor related protein complex 3 subunit mu 2
Transcript:
RefSeq NM_006803.2 (NON-CURRENT)
Match location:
Position 1263 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000065289)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCTGGATATGTATGGAGAA
Hairpin Sequence:
5'-CCGG-CGTCTGGATATGTATGGAGAA-CTCGAG-TTCTCCATACATATCCAGACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCTGGATATGTATGGAGAACTCGAGTTCTCCATACATATCCAGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCTGGATATGTATGGAGAACTCGAGTTCTCCATACATATCCAGACG-3'

Other clones with same target sequence:

(none)