Construct: shRNA TRCN0000065303

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006815.2-450s1c1
DNA Barcode:
GCTGTAAAGCACGAACAGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMED2 (10959)
Gene Description:
transmembrane p24 trafficking protein 2
Transcript:
RefSeq NM_006815.2 (NON-CURRENT)
Match location:
Position 450 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000065303)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTAAAGCACGAACAGGAA
Hairpin Sequence:
5'-CCGG-GCTGTAAAGCACGAACAGGAA-CTCGAG-TTCCTGTTCGTGCTTTACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTAAAGCACGAACAGGAACTCGAGTTCCTGTTCGTGCTTTACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTAAAGCACGAACAGGAACTCGAGTTCCTGTTCGTGCTTTACAGC-3'

Other clones with same target sequence:

  1. TRCN0000289256