Construct: shRNA TRCN0000065322

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002267.2-920s1c1
DNA Barcode:
CCACATCAGAATGTTTGTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KPNA3 (3839)
Gene Description:
karyopherin subunit alpha 3
Transcript:
RefSeq NM_002267.2 (NON-CURRENT)
Match location:
Position 920 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000065322)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACATCAGAATGTTTGTGAA
Hairpin Sequence:
5'-CCGG-CCACATCAGAATGTTTGTGAA-CTCGAG-TTCACAAACATTCTGATGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACATCAGAATGTTTGTGAACTCGAGTTCACAAACATTCTGATGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACATCAGAATGTTTGTGAACTCGAGTTCACAAACATTCTGATGTGG-3'

Other clones with same target sequence:

  1. TRCN0000286474