Construct: shRNA TRCN0000065326

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144678.2-831s1c1
DNA Barcode:
GTCAACGATGACCTCAACAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TOM1L2 (146691)
Gene Description:
target of myb1 like 2 membrane trafficking protein
Transcript:
RefSeq NM_144678.2 (NON-CURRENT)
Match location:
Position 831 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000065326)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCAACGATGACCTCAACAAT
Hairpin Sequence:
5'-CCGG-GTCAACGATGACCTCAACAAT-CTCGAG-ATTGTTGAGGTCATCGTTGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCAACGATGACCTCAACAATCTCGAGATTGTTGAGGTCATCGTTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCAACGATGACCTCAACAATCTCGAGATTGTTGAGGTCATCGTTGAC-3'

Other clones with same target sequence:

(none)