Construct: shRNA TRCN0000065350
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_355508.2-1661s1c1
- DNA Barcode:
- GCAATCTATTAGTGTTCCTTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- LOC381539 (381539)
- Gene Description:
- similar to zinc finger, FYVE domain containing 9 isoform 2; MADH-interacting protein; smad anchor for receptor activation; mothers against decapentaplegic homolog (Drosophila) interacting protein; MAD homolog interacting protein; receptor activatio...
- Transcript:
- RefSeq XM_355508.2 (NON-CURRENT)
- Match location:
- Position 1661 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000065350)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCAATCTATTAGTGTTCCTTT
- Hairpin Sequence:
-
5'-CCGG-GCAATCTATTAGTGTTCCTTT-CTCGAG-AAAGGAACACTAATAGATTGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCAATCTATTAGTGTTCCTTTCTCGAGAAAGGAACACTAATAGATTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCAATCTATTAGTGTTCCTTTCTCGAGAAAGGAACACTAATAGATTGC-3'
Other clones with same target sequence:
(none)
