Construct: shRNA TRCN0000065351

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_355508.2-661s1c1
DNA Barcode:
CGGAGAAACAAATGGATGCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC381539 (381539)
Gene Description:
similar to zinc finger, FYVE domain containing 9 isoform 2; MADH-interacting protein; smad anchor for receptor activation; mothers against decapentaplegic homolog (Drosophila) interacting protein; MAD homolog interacting protein; receptor activatio...
Transcript:
RefSeq XM_355508.2 (NON-CURRENT)
Match location:
Position 661 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000065351)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAGAAACAAATGGATGCTT
Hairpin Sequence:
5'-CCGG-CGGAGAAACAAATGGATGCTT-CTCGAG-AAGCATCCATTTGTTTCTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAGAAACAAATGGATGCTTCTCGAGAAGCATCCATTTGTTTCTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAGAAACAAATGGATGCTTCTCGAGAAGCATCCATTTGTTTCTCCG-3'

Other clones with same target sequence:

(none)