Construct: shRNA TRCN0000065394

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133223.2-348s1c1
DNA Barcode:
CTCTCCTATCCTCAAACAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rac3 (170758)
Gene Description:
RAS-related C3 botulinum substrate 3
Transcript:
RefSeq NM_133223.2 (NON-CURRENT)
Match location:
Position 348 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000065394)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCTCCTATCCTCAAACAGAT
Hairpin Sequence:
5'-CCGG-CTCTCCTATCCTCAAACAGAT-CTCGAG-ATCTGTTTGAGGATAGGAGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTCCTATCCTCAAACAGATCTCGAGATCTGTTTGAGGATAGGAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTCCTATCCTCAAACAGATCTCGAGATCTGTTTGAGGATAGGAGAG-3'

Other clones with same target sequence:

(none)