Construct: shRNA TRCN0000065467

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008374.1-940s1c1
DNA Barcode:
CCATCCTCTCTACAGTGTGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Il9r (16199)
Gene Description:
interleukin 9 receptor
Transcript:
RefSeq NM_008374.1 (NON-CURRENT)
Match location:
Position 940 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000065467)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCCTCTCTACAGTGTGTA
Hairpin Sequence:
5'-CCGG-CCATCCTCTCTACAGTGTGTA-CTCGAG-TACACACTGTAGAGAGGATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCCTCTCTACAGTGTGTACTCGAGTACACACTGTAGAGAGGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCCTCTCTACAGTGTGTACTCGAGTACACACTGTAGAGAGGATGG-3'

Other clones with same target sequence:

(none)