Construct: shRNA TRCN0000065560
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_013793.2-497s1c1
- DNA Barcode:
- GCTGCCAGAATTCCAGCTTAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Klra15 (27423)
- Gene Description:
- killer cell lectin-like receptor, subfamily A, member 15
- Transcript:
- RefSeq NM_013793.2 (CURRENT)
- Match location:
- Position 497 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000065560)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTGCCAGAATTCCAGCTTAT
- Hairpin Sequence:
-
5'-CCGG-GCTGCCAGAATTCCAGCTTAT-CTCGAG-ATAAGCTGGAATTCTGGCAGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTGCCAGAATTCCAGCTTATCTCGAGATAAGCTGGAATTCTGGCAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTGCCAGAATTCCAGCTTATCTCGAGATAAGCTGGAATTCTGGCAGC-3'
Other clones with same target sequence:
(none)
