Construct: shRNA TRCN0000065564

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008947.1-1236s1c1
DNA Barcode:
CGCAGAATGAAAGTAACAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Psmc1 (19179)
Gene Description:
protease (prosome, macropain) 26S subunit, ATPase 1
Transcript:
RefSeq NM_008947.1 (NON-CURRENT)
Match location:
Position 1236 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000065564)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCAGAATGAAAGTAACAAAT
Hairpin Sequence:
5'-CCGG-CGCAGAATGAAAGTAACAAAT-CTCGAG-ATTTGTTACTTTCATTCTGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCAGAATGAAAGTAACAAATCTCGAGATTTGTTACTTTCATTCTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCAGAATGAAAGTAACAAATCTCGAGATTTGTTACTTTCATTCTGCG-3'

Other clones with same target sequence:

(none)