Construct: shRNA TRCN0000065608

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007555.2-2133s1c1
DNA Barcode:
GCTATGTTTATGACCACAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bmp5 (12160)
Gene Description:
bone morphogenetic protein 5
Transcript:
RefSeq NM_007555.2 (NON-CURRENT)
Match location:
Position 2133 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000065608)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTATGTTTATGACCACAATA
Hairpin Sequence:
5'-CCGG-GCTATGTTTATGACCACAATA-CTCGAG-TATTGTGGTCATAAACATAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTATGTTTATGACCACAATACTCGAGTATTGTGGTCATAAACATAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTATGTTTATGACCACAATACTCGAGTATTGTGGTCATAAACATAGC-3'

Other clones with same target sequence:

(none)