Construct: shRNA TRCN0000065613

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013584.1-1836s1c1
DNA Barcode:
GCAGAGATACAGCTTAGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lifr (16880)
Gene Description:
leukemia inhibitory factor receptor
Transcript:
RefSeq NM_013584.1 (NON-CURRENT)
Match location:
Position 1836 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000065613)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAGATACAGCTTAGTAAA
Hairpin Sequence:
5'-CCGG-GCAGAGATACAGCTTAGTAAA-CTCGAG-TTTACTAAGCTGTATCTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAGATACAGCTTAGTAAACTCGAGTTTACTAAGCTGTATCTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAGATACAGCTTAGTAAACTCGAGTTTACTAAGCTGTATCTCTGC-3'

Other clones with same target sequence:

(none)