Construct: shRNA TRCN0000065615
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_013584.1-734s1c1
- DNA Barcode:
- GCCTCATTTCTCCGGTTACAA
Original Target:
- Taxon:
- Mus musculus (mouse)
- Gene:
- Lifr (16880)
- Gene Description:
- leukemia inhibitory factor receptor
- Transcript:
- RefSeq NM_013584.1 (NON-CURRENT)
- Match location:
- Position 734 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this shRNA:
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Sequence Information
- Target Sequence:
- GCCTCATTTCTCCGGTTACAA
- Hairpin Sequence:
- 5'-CCGG-GCCTCATTTCTCCGGTTACAA-CTCGAG-TTGTAACCGGAGAAATGAGGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCTCATTTCTCCGGTTACAACTCGAGTTGTAACCGGAGAAATGAGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCTCATTTCTCCGGTTACAACTCGAGTTGTAACCGGAGAAATGAGGC-3'