Construct: shRNA TRCN0000065701

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009399.2-1394s1c1
DNA Barcode:
GTCCCTGAAATGTGGACCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tnfrsf11a (21934)
Gene Description:
tumor necrosis factor receptor superfamily, member 11a, NFKB activator
Transcript:
RefSeq NM_009399.2 (NON-CURRENT)
Match location:
Position 1394 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000065701)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCCCTGAAATGTGGACCATT
Hairpin Sequence:
5'-CCGG-GTCCCTGAAATGTGGACCATT-CTCGAG-AATGGTCCACATTTCAGGGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCCCTGAAATGTGGACCATTCTCGAGAATGGTCCACATTTCAGGGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCCCTGAAATGTGGACCATTCTCGAGAATGGTCCACATTTCAGGGAC-3'

Other clones with same target sequence:

(none)