Construct: shRNA TRCN0000065737

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008520.1-2884s1c1
DNA Barcode:
GACAGCGTATTGGCTACCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ltbp3 (16998)
Gene Description:
latent transforming growth factor beta binding protein 3
Transcript:
RefSeq NM_008520.1 (NON-CURRENT)
Match location:
Position 2884 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000065737)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACAGCGTATTGGCTACCAAT
Hairpin Sequence:
5'-CCGG-GACAGCGTATTGGCTACCAAT-CTCGAG-ATTGGTAGCCAATACGCTGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACAGCGTATTGGCTACCAATCTCGAGATTGGTAGCCAATACGCTGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACAGCGTATTGGCTACCAATCTCGAGATTGGTAGCCAATACGCTGTC-3'

Other clones with same target sequence:

  1. TRCN0000301492