Construct: shRNA TRCN0000065806

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_207773.3-2933s1c1
DNA Barcode:
CCCTTATAGGACTCTCCCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC280413 (280413)
Gene Description:
similar to integrin alpha 10 subunit
Transcript:
RefSeq XM_207773.3 (NON-CURRENT)
Match location:
Position 2933 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000065806)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTATAGGACTCTCCCAAT
Hairpin Sequence:
5'-CCGG-CCCTTATAGGACTCTCCCAAT-CTCGAG-ATTGGGAGAGTCCTATAAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTATAGGACTCTCCCAATCTCGAGATTGGGAGAGTCCTATAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTATAGGACTCTCCCAATCTCGAGATTGGGAGAGTCCTATAAGGG-3'

Other clones with same target sequence:

(none)