Construct: shRNA TRCN0000066002

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013565.2-2668s1c1
DNA Barcode:
GCCTCGCTCAGCTTAATGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Itga3 (16400)
Gene Description:
integrin alpha 3
Transcript:
RefSeq NM_013565.2 (NON-CURRENT)
Match location:
Position 2668 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000066002)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCGCTCAGCTTAATGAAT
Hairpin Sequence:
5'-CCGG-GCCTCGCTCAGCTTAATGAAT-CTCGAG-ATTCATTAAGCTGAGCGAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCGCTCAGCTTAATGAATCTCGAGATTCATTAAGCTGAGCGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCGCTCAGCTTAATGAATCTCGAGATTCATTAAGCTGAGCGAGGC-3'

Other clones with same target sequence:

(none)