Construct: shRNA TRCN0000066041

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007654.1-733s1c1
DNA Barcode:
CCATGCGGATGGATTCCATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cd72 (12517)
Gene Description:
CD72 antigen
Transcript:
RefSeq NM_007654.1 (NON-CURRENT)
Match location:
Position 733 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000066041)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGCGGATGGATTCCATAT
Hairpin Sequence:
5'-CCGG-CCATGCGGATGGATTCCATAT-CTCGAG-ATATGGAATCCATCCGCATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGCGGATGGATTCCATATCTCGAGATATGGAATCCATCCGCATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGCGGATGGATTCCATATCTCGAGATATGGAATCCATCCGCATGG-3'

Other clones with same target sequence:

(none)