Construct: shRNA TRCN0000066277

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033523.2-932s1c1
DNA Barcode:
GCTACGGACAGTTCTTCTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spred2 (114716)
Gene Description:
sprouty-related, EVH1 domain containing 2
Transcript:
RefSeq NM_033523.2 (NON-CURRENT)
Match location:
Position 932 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000066277)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTACGGACAGTTCTTCTAAT
Hairpin Sequence:
5'-CCGG-GCTACGGACAGTTCTTCTAAT-CTCGAG-ATTAGAAGAACTGTCCGTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTACGGACAGTTCTTCTAATCTCGAGATTAGAAGAACTGTCCGTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTACGGACAGTTCTTCTAATCTCGAGATTAGAAGAACTGTCCGTAGC-3'

Other clones with same target sequence:

  1. TRCN0000303154