Construct: shRNA TRCN0000066317

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033524.2-414s1c1
DNA Barcode:
GTCCCTCATCAGGAAGAGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spred1 (114715)
Gene Description:
sprouty protein with EVH-1 domain 1, related sequence
Transcript:
RefSeq NM_033524.2 (NON-CURRENT)
Match location:
Position 414 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000066317)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCCCTCATCAGGAAGAGAAT
Hairpin Sequence:
5'-CCGG-GTCCCTCATCAGGAAGAGAAT-CTCGAG-ATTCTCTTCCTGATGAGGGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCCCTCATCAGGAAGAGAATCTCGAGATTCTCTTCCTGATGAGGGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCCCTCATCAGGAAGAGAATCTCGAGATTCTCTTCCTGATGAGGGAC-3'

Other clones with same target sequence:

(none)