Construct: shRNA TRCN0000066426

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009735.2-225s1c1
DNA Barcode:
CCAAATGCTGAAGAACGGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
B2m (12010)
Gene Description:
beta-2 microglobulin
Transcript:
RefSeq NM_009735.2 (NON-CURRENT)
Match location:
Position 225 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000066426)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAATGCTGAAGAACGGGAA
Hairpin Sequence:
5'-CCGG-CCAAATGCTGAAGAACGGGAA-CTCGAG-TTCCCGTTCTTCAGCATTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAATGCTGAAGAACGGGAACTCGAGTTCCCGTTCTTCAGCATTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAATGCTGAAGAACGGGAACTCGAGTTCCCGTTCTTCAGCATTTGG-3'

Other clones with same target sequence:

(none)