Construct: shRNA TRCN0000066559

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011192.2-272s1c1
DNA Barcode:
GCAGAAGACTTGGTGGCAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Psme3 (19192)
Gene Description:
proteaseome (prosome, macropain) activator subunit 3 (PA28 gamma, Ki)
Transcript:
RefSeq NM_011192.2 (NON-CURRENT)
Match location:
Position 272 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000066559)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAAGACTTGGTGGCAAAT
Hairpin Sequence:
5'-CCGG-GCAGAAGACTTGGTGGCAAAT-CTCGAG-ATTTGCCACCAAGTCTTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAAGACTTGGTGGCAAATCTCGAGATTTGCCACCAAGTCTTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAAGACTTGGTGGCAAATCTCGAGATTTGCCACCAAGTCTTCTGC-3'

Other clones with same target sequence:

  1. TRCN0000335512