Construct: shRNA TRCN0000066628

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007644.2-1835s1c1
DNA Barcode:
GCCTGTCAAGAAGGAAACATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Scarb2 (12492)
Gene Description:
scavenger receptor class B, member 2
Transcript:
RefSeq NM_007644.2 (NON-CURRENT)
Match location:
Position 1835 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000066628)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGTCAAGAAGGAAACATA
Hairpin Sequence:
5'-CCGG-GCCTGTCAAGAAGGAAACATA-CTCGAG-TATGTTTCCTTCTTGACAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGTCAAGAAGGAAACATACTCGAGTATGTTTCCTTCTTGACAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGTCAAGAAGGAAACATACTCGAGTATGTTTCCTTCTTGACAGGC-3'

Other clones with same target sequence:

  1. TRCN0000312028